Evaluation of a novel multiplex qPCR method for rapid detection and quantification of pathogens associated with calf diarrhoea
Potjamas Pansri,Birgitta Svensmark,Gang Liu,Stig Milan Thamsborg,Egle Kudirkiene,Henrik Vedel Nielsen,Nicole Bakkegård Goecke,John Elmerdahl Olsen
Abstract
AimsDiarrhoea is a common health problem in calves and a main reason for use of antimicrobials. It is associated with several bacterial, viral and parasitic pathogens, most of which are commonly present in healthy animals. Methods, which quantify the causative agents, may therefore improve confidence in associating a pathogen to the disease. This study evaluated a novel commercially available, multiplex quantitative polymerase chain reaction (qPCR) assay (Enterit4Calves) for detection and quantification of pathogens associated with calf-diarrhoea.
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