Pneumo 4 a new qPCR test

Experience with a new PCR-assay for diagnosis of bacterial infections in calves

SOURSE:Potjamas Pansri, DNA Diagnostic A/S, Risskov Denmark. Egle Kudirkiene, Department of veterinary and Animal Science, University of Copenhagen. John E. Olsen, LVK Veterinary Cattle Proctice, Denmark. Jørgen Katholm,
SOURSE:Potjamas Pansri, DNA Diagnostic A/S, Risskov Denmar
ABSTRACT: This project is the first from the GUDP project Diagnostic tests for veterinary practice (VetDiagnostics).
In this part, we have developed a fast qPCR test also to be used in private veterinary practice, for detection of pathogens associate with pneumonia in calf.
We have designed primers and probes for detection of Mannheimia hae molytica, Pasterurellamultocida, Histophilus sommi and mycoplasma bovis. We tested these primers for respectively 15,16, 10 and 3 different target isolates and all turned out to be correct positive. Then we tested all primers for 135 different non-target isolates. All were negative, among these isolates we also tested very related subtypes of Mannheimia and Pasteurella.

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