Validation paper

Evaluation of novel multiplex qPCR assays for diagnosis of pathogens associated with the bovine respiratory disease complex

P. Pansria (A), J. Katholm (A), K.M. Krogh (B), A.K. Aagaard (C), L.M.B. Schmidt (C), E. Kudirkiene (C), L.E. Larsen (D), J.E. Olsen (C)

(A) DNA Diagnostic, Risskov, Denmark
(B) LVK Veterinary Cattle Practice, Hobro, Denmark
(C) Department of Veterinary and Animal Science, University of Copenhagen, Copenhagen Denmark
(D) National Veterinary Laboratory, Technical University of Denmark, Lyngby, Denmark

Bovine respiratory disease complex is the most common disease requiring the use of antimicrobials in industrial calf production worldwide.

Pathogenic bacteria (Mannheimia haemolytica (Mh), Pasteurella multocida (Pm), Histophilus somni (Hs), and Mycoplasma bovis) and a range of viruses (bovine respiratory syncytial virus, bovine coronavirus, bovine parainfluenza virus type 3, bovine viral diarrhea virus and bovine herpesvirus type 1) are associated with this complex.

As most of these pathogens can be present in healthy and diseased calves, simple detection of their presence in diseased calves carries low predictive value. In other multi-agent diseases of livestock, quantification of pathogens has added substantially to the predictive value of microbiological diagnosis.

The aim of this study was to evaluate the ability of two recently developed quantitative PCR (qPCR) kits (Pneumo4B and Pneumo4V) to detect and quantify these bacterial and viral pathogens, respectively.

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